FastQCFastQC Report
Sun 5 May 2024
EGAF00003593515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44122676
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTA523897111.873647464174656Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG32029087.259097340333574Illumina Paired End PCR Primer 2 (100% over 31bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC3933820.8915642378535699Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCT2021260.4581000481475784Illumina Paired End Sequencing Primer 2 (100% over 25bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGC543670.12321782114937906Illumina Paired End Sequencing Primer 2 (100% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATTCATCTCGTA466730.10578007553304336Illumina Paired End Sequencing Primer 2 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)