FastQCFastQC Report
Sun 14 May 2023
EGAF00003593515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44122676
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41818749.477834028017702Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCG29799736.753835601449014Illumina Paired End PCR Primer 2 (97% over 37bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTC3666700.8310239388018985Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG1716340.3889927256451989Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC1183930.2683268802644699Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT938980.2128112084588886Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA732230.16595321643682717Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA704890.15975685608914564Illumina Paired End PCR Primer 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)