FastQCFastQC Report
Thu 2 May 2024
EGAF00003593516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37292162
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTA31183298.361888484770606Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG6134541.644994462911536Illumina Paired End PCR Primer 2 (100% over 31bp)
GATTGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTA722860.19383697839776626Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGC551360.14784876242895223Illumina Paired End PCR Primer 2 (97% over 35bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC439330.11780759721037358Illumina Paired End PCR Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGAATCTCGTA412690.11066400494559687Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)