FastQCFastQC Report
Sat 13 May 2023
EGAF00003593516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37292162
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22456876.021873979845953Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCG5522641.4809117261691613Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG1025750.2750577990088105Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC949440.2545950540491592Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT923660.2476820732463835Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA540300.1448829917664736Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA472860.1267987626997866Illumina Paired End PCR Primer 2 (97% over 38bp)
GATTGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA434660.11655532334113533Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA401240.1075936546666294Illumina Paired End PCR Primer 2 (97% over 38bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTC395350.10601423430478502Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)