FastQCFastQC Report
Mon 6 Mar 2023
EGAF00003599888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165532819
Sequences flagged as poor quality0
Sequence length41-56
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6243100.37715179610394967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGG3083670.18628753008791568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGG2315780.1398985418112163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAG1667280.100722020568018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)