FastQCFastQC Report
Mon 6 Feb 2023
EGAF00003599893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544035784
Sequences flagged as poor quality0
Sequence length20-21
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNN361735946.649120345363165No Hit
ATGGGGCTAAACCTAGCCCCA74412811.3677925641744184No Hit
ATGGGGGCGCAATAGATATAG66007661.2132962930247249No Hit
ATGGGGACAACCTTAGCCAAA31913330.586603509154464No Hit
ATGGGGGCGACAAACCTACCG20104800.36954922068141016No Hit
ATGGGTAGAATCTTAGTTCAA18135140.33334461690483214No Hit
ATGGGGCGCAATAGATATAGT16928180.3111593115352868No Hit
ATGGGAGGAATTTGAAGTAGA14404020.2647623634992363No Hit
GGGGCGCAATAGATATAGTA11713980.21531635132294902No Hit
GGGGCAGTTCGGCGGTCCCG11530300.21194010282235407No Hit
ATGGGCTGATGTTCGCCGAC10723510.19711037978340043No Hit
ATGGGCAGTTGACACAAAATA10174660.18702188898662594No Hit
GGGCTAAACCTAGCCCCAAA9348760.17184090228888327No Hit
GTGGGGGCGCAATAGATATAG9289350.17074887853332824No Hit
GGGGCTAAACCTAGCCCCAAA8949470.16450149536487108No Hit
GTGGGGCTAAACCTAGCCCCA8592050.15793170693345424No Hit
ATGGGATGACCCACCAATCA6229090.11449779928446767No Hit
ATGGGGGAGCTTTAATTTATT5914190.10870957708914236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)