FastQCFastQC Report
Fri 24 Mar 2023
EGAF00003599906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136556029
Sequences flagged as poor quality0
Sequence length26-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGT17195381.2592179287814527Clontech Universal Primer Mix Long (96% over 25bp)
GGTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3383450.247770092963087Clontech SMART CDS Primer II A (96% over 26bp)
GTCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2956610.21651259352305857Clontech SMART CDS Primer II A (96% over 26bp)
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAAT2757640.20194201751429075No Hit
GTTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2607850.19097289362449169Clontech SMART CDS Primer II A (96% over 26bp)
GTAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2528640.18517234416651057Clontech SMART CDS Primer II A (100% over 26bp)
GGCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2415870.1769141954179116Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2378740.17419516497510337Clontech SMART CDS Primer II A (96% over 26bp)
GCTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2329460.17058638985467275Clontech SMART CDS Primer II A (96% over 26bp)
GGGGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2267970.16608347625574263Clontech SMART CDS Primer II A (96% over 26bp)
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2265100.16587330611378573No Hit
GATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2233200.16353726864743556Clontech SMART CDS Primer II A (96% over 26bp)
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTA2098810.153695886982771No Hit
ATCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT2087120.1528398281118734Clontech SMART CDS Primer II A (96% over 26bp)
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT1939200.14200764434941207No Hit
CCTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1935480.14173522869502891Clontech SMART CDS Primer II A (96% over 26bp)
ACCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1873760.13721547219273636Clontech SMART CDS Primer II A (96% over 26bp)
GGAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1854640.13581531431321864Clontech SMART CDS Primer II A (100% over 26bp)
ACTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1769690.12959442457132375Clontech SMART CDS Primer II A (96% over 26bp)
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACA1743560.12768092428932595No Hit
GCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1738690.1273242941181308Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1721460.12606254096624325Clontech SMART CDS Primer II A (96% over 26bp)
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACC1697160.12428305161099845No Hit
GACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1677590.1228499402249021Clontech SMART CDS Primer II A (96% over 26bp)
AGTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1601960.11731155421925751Clontech SMART CDS Primer II A (96% over 26bp)
CTCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1593550.11669568979631065Clontech SMART CDS Primer II A (96% over 26bp)
GCGGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1532420.11221913900264337Clontech SMART CDS Primer II A (96% over 26bp)
ATAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1526310.1117717036133205Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1508150.11044184654783716Clontech SMART CDS Primer II A (100% over 26bp)
AGCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1497070.10963045798585722Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1462520.10710036098076636Clontech SMART CDS Primer II A (96% over 26bp)
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC1453200.10641785724451609No Hit
ATGGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1449000.10611029118311577Clontech SMART CDS Primer II A (96% over 26bp)
ATTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1416530.10373251260843269Clontech SMART CDS Primer II A (96% over 26bp)
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAA1398030.10237775733797884No Hit
TCTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1381950.10120021870290326Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)