FastQCFastQC Report
Tue 24 Jan 2023
EGAF00003599912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357056118
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCC36637841.0261087306169614Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGTA8853980.2479716647790362Illumina RNA PCR Primer (100% over 25bp)
GCCCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCG6452540.18071501018223696Clontech SMART CDS Primer II A (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5480500.1534912783653801No Hit
CTCCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCG4981360.13951196321470116Clontech SMART CDS Primer II A (96% over 26bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGG4900260.13724061157243636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)