FastQCFastQC Report
Wed 29 Mar 2023
EGAF00003599923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230677117
Sequences flagged as poor quality0
Sequence length20-21
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNN2848332112.347701137603519No Hit
ATGGGGCTAAACCTAGCCCCA41463201.7974561386598222No Hit
ATGGGGGCGCAATAGATATAG39317951.7044581842940234No Hit
ATGGGGACAACCTTAGCCAAA25402611.10121932900696No Hit
ATGGGTAGAATCTTAGTTCAA16961740.7353022363288856No Hit
ATGGGGCGCAATAGATATAGT14916970.6466601539848446No Hit
ATGGGGGCGACAAACCTACCG11272330.4886626877688956No Hit
ATGGGAGGAATTTGAAGTAGA11023260.4778653445716508No Hit
ATGGGCTGATGTTCGCCGAC10283140.44578067099737506No Hit
ATGGGCTAAACCTAGCCCCAA9622700.4171501761919454No Hit
ACCCGTCTATGTAGCAAAATA7555410.3275318374990789No Hit
ATGGGACAACCTTAGCCAAA7025720.30456943850221607No Hit
ATGGGCAGTTGACACAAAATA6780670.29394636486635123No Hit
ATGGGTCATTATTACCCTCA4979660.21587143383623958No Hit
ATGGGATGACCCACCAATCA3860870.16737117448888528No Hit
ATGGGGGAGCTTTAATTTATT3685610.15977354182036183No Hit
ATGGGGGAAGGCGCTTTGTGA3181480.1379191851092885No Hit
ATGGGCGCGACCTCAGATCAG3156200.13682328100190363No Hit
ATGGGGAGGAGAATGTTTTCA2995780.12986897178882292No Hit
ATGGGATAGGTTTGGTCCTAG2951360.12794333648621073No Hit
ATGGGGGAAGTATGTAGGAGT2550540.11056753410005553No Hit
ATGGGCTAAATCCCCTTGTAA2472360.10717838128694837No Hit
ATGGGCATATTTCACCTCCG2385800.10342595013444701No Hit
ATGGGTGCTAAACCTAGCCC2332370.10110972559103035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)