FastQCFastQC Report
Tue 24 Jan 2023
EGAF00003599930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325515468
Sequences flagged as poor quality0
Sequence length26-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGT39244601.2056139832961794Clontech Universal Primer Mix Long (96% over 25bp)
GTCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT7648890.2349777737751006Clontech SMART CDS Primer II A (96% over 26bp)
GTTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT6630860.2037033767009806Clontech SMART CDS Primer II A (96% over 26bp)
GGTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT5875570.18050048546387357Clontech SMART CDS Primer II A (96% over 26bp)
GGCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT5763060.1770441212950286Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT5508640.16922821007080377Clontech SMART CDS Primer II A (96% over 26bp)
GCTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT5464240.16786421958909797Clontech SMART CDS Primer II A (96% over 26bp)
GTAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4917110.15105610895270882Clontech SMART CDS Primer II A (100% over 26bp)
GGGGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4523570.1389663608858059Clontech SMART CDS Primer II A (96% over 26bp)
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAAT4421600.13583379085383435No Hit
GGAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4391500.13490910361285813Clontech SMART CDS Primer II A (100% over 26bp)
ATCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4299830.1320929547962372Clontech SMART CDS Primer II A (96% over 26bp)
GCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4271710.13122909415782355Clontech SMART CDS Primer II A (96% over 26bp)
GATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT4211270.12937234675434842Clontech SMART CDS Primer II A (96% over 26bp)
GACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3856420.11847117507792287Clontech SMART CDS Primer II A (96% over 26bp)
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC3798950.11670566757829154No Hit
CCTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3590990.11031703107884261Clontech SMART CDS Primer II A (96% over 26bp)
CTCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3584820.11012748555469566Clontech SMART CDS Primer II A (96% over 26bp)
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTA3539190.1087257088501859No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAAA3462550.10637128924392619No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3445240.105839517279099Clontech SMART CDS Primer II A (96% over 26bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3334430.10243537797104008Clontech SMART CDS Primer II A (96% over 26bp)
TCTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT3314920.10183602089225449Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)