FastQCFastQC Report
Thu 26 May 2022
EGAF00003599932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590834436
Sequences flagged as poor quality0
Sequence length26-61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGC87343071.4783002593978798Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCG67693741.1457311198428521Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGTA43333390.7334269527918985Illumina RNA PCR Primer (100% over 25bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGG18802650.3182388983163466No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTA11179900.1892222138521391No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGG5974460.10111902143767396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)