FastQCFastQC Report
Wed 8 Feb 2023
EGAF00003599933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626174197
Sequences flagged as poor quality0
Sequence length20-21
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTGTCTCTTATACACATC99992041.596872571227971No Hit
CTGTCTCTTATACACATCTCC55165780.8809973369119839No Hit
ATGGGCTGTCTCTTATACACA43324260.6918882989360866No Hit
ATGGGCGCGACCTCAGATCAG21373400.34133313225616674No Hit
ATGGGGCTAAACCTAGCCCCA13143930.20990852166972956No Hit
ATGGGGGCGCAATAGATATAG6619420.10571211703889484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)