FastQCFastQC Report
Thu 9 Feb 2023
EGAF00003599936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177386905
Sequences flagged as poor quality0
Sequence length20-61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCC8639710.4870545545625254Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGTA8322380.4691654099269616Illumina RNA PCR Primer (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCG6914900.3898202068523604Clontech SMART CDS Primer II A (96% over 26bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGG1926480.10860328162329681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)