FastQCFastQC Report
Mon 27 Mar 2023
EGAF00003599945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212113744
Sequences flagged as poor quality0
Sequence length21
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGCTAAACCTAGCCCCA13145760.6197505051817859No Hit
ATGGGGGCGCAATAGATATAG12571680.5926857808893327No Hit
ATGCTGTCTCTTATACACATC9639940.4544703147571616No Hit
ATGGGCTGTCTCTTATACACA8155140.38447013598515334No Hit
ATGGGCTGATGTTCGCCGACC6895370.325078887863108No Hit
ATGGGGACAACCTTAGCCAAA5399080.2545370185913083No Hit
ATGGGAATAACGCCGCCGCAT4969200.2342705336434965No Hit
ATGGGGCAGTTCGGCGGTCCC4298240.20263844854862398No Hit
CTGTCTCTTATACACATCTCC3966450.1869963692687448No Hit
ATGGGATGACCCACCAATCAC3945790.1860223635484931No Hit
GTGGGGGCGCAATAGATATAG3636620.17144669324209375No Hit
ATGGGGCGCAATAGATATAGT3395630.1600853361015588No Hit
ATGGGGGCGACAAACCTACCG3309350.15601770717884267No Hit
ATGGGAGGAATTTGAAGTAGA3128140.1474746492617659No Hit
ATGGGGGCAGTTCGGCGGTCC2935390.13838754361904998No Hit
ATGGGTAGAATCTTAGTTCAA2867490.1351864309179324No Hit
GTGGGGCTAAACCTAGCCCCA2855880.13463908307610656No Hit
GTGGGGCAGTTCGGCGGTCCC2623560.12368646889755527No Hit
ATGGGATGGCACATGCAGCGC2501100.11791315135147488No Hit
ATGGGGAATAATTGCAATCCC2413520.11378423455671972No Hit
GTGGGCTGATGTTCGCCGACC2410820.11365694436094625No Hit
ATGGGAGATGCACAGGAGGAG2183800.10295419612224657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)