FastQCFastQC Report
Wed 6 Sep 2023
EGAF00003599951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200123156
Sequences flagged as poor quality0
Sequence length20-21
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNN112848165.638935656201624No Hit
ATGGGGCTAAACCTAGCCCCA25992861.298843198335329No Hit
ATGGGGGCGCAATAGATATAG25586241.278524710054043No Hit
ATGGGGACAACCTTAGCCAAA15089480.7540096959094529No Hit
ATGGGTAGAATCTTAGTTCAA9538330.4766230050859282No Hit
ATGGGGCGCAATAGATATAGT7465500.37304528617367994No Hit
ATGGGGGCGACAAACCTACCG6761290.33785645475229265No Hit
ATGGGCTGATGTTCGCCGAC6338550.31673246248425146No Hit
ATGGGAGGAATTTGAAGTAGA5279470.2638110504313654No Hit
GTGGGGGCGCAATAGATATAG5265730.2631244732118856No Hit
ACCCGTCTATGTAGCAAAATA4151170.20743076828150764No Hit
GTGGGGCTAAACCTAGCCCCA4136080.2066767326015986No Hit
ATGGGACAACCTTAGCCAAA3297880.16479252405953462No Hit
ATGGGCAGTTGACACAAAATA3205040.16015338075120103No Hit
ATGGGAATAACGCCGCCGCAT3124720.15613985220181117No Hit
GTGGGCTGATGTTCGCCGAC2302100.11503416426233055No Hit
GTGGGGACAACCTTAGCCAAA2013450.10061054603796074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)