FastQCFastQC Report
Wed 22 Feb 2023
EGAF00003599954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003599954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142150271
Sequences flagged as poor quality0
Sequence length45-61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGC3690440.2596154037581821Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCG2759280.19411007665261504Clontech SMART CDS Primer II A (96% over 26bp)
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTG1936650.13623962771059367No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTA1923100.13528641109660636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)