FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003600013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11980356
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG213800.17845880372837TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTATG171590.14322612783793737TruSeq Adapter, Index 6 (97% over 35bp)
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT168960.14103086753014685No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT157860.13176570045163935No Hit
CAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTCTTCATTATAGCTATG136660.11407006603142678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)