FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003600014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11980356
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC336930.28123538232085926Illumina Single End PCR Primer 1 (96% over 33bp)
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT175170.14621435289569026No Hit
CAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTCTTCATTATAGCTATG135060.11273454645254281No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT126020.1051888608318484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)