FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003600015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13819423
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG544060.3936922692068982TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTATG465010.33649017039278706TruSeq Adapter, Index 6 (97% over 35bp)
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT362030.26197186380357557No Hit
TATGTCTGCTGATAATGAAAATGATGACTCTGTCTCAGATGATAACTTTA259230.18758380867276442No Hit
AGAGGCCCAGAGAGAGAGGTGGTGCTGAGTGTGCAAATCCCCCATCTACT236470.17111423537726575No Hit
ACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCT235290.17026036470553077No Hit
ACAGTGTGTACCTTCCAGAACGGTCAACCATGCAGAGTGATGGGAGAGTC233700.1691098101563285No Hit
ACTCCTGTGATGGGCGAGAGGAAGCAGCGATGGGCCGGGCCCCTCCTCCC194440.14070051984080667No Hit
CTCTAGTCTAGCCCCCTGCCCCTCAAGCCCACTCTTGCCCCAAGGCCTGG191580.1386309688906693No Hit
CGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCCAGAGC189480.13711136854266637No Hit
GGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATA184770.13370312204785975No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT184610.13358734297372618No Hit
CAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTCTTCATTATAGCTATG182090.13176382255612265No Hit
GCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGC178670.12928904484651782No Hit
GGGCAGATGGGCAAGACACCTCCAGGTCCTGGCCTGGGCCCTGCTCCTGA170540.1234060206421064No Hit
GAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGA169850.12290672338490544No Hit
CCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAAG169260.12247978804903793No Hit
CAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGC166490.12047536282810072No Hit
CAAAGTATTATTACCTGTCCCGAGGCAGCTCCCAGCGAGGGTCTTCGGGA165250.11957807500356564No Hit
AGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGG164660.11915113966769814No Hit
GCAAGGACAGAAGCATCACTTACACTTCAACATCTTCACAGCCACTTTGG161590.11692962868276048No Hit
GGCCCACGAAGACTGGTGCCATGATTACCTTCAAGATCTGGACATAAGGC154500.11179916846021719No Hit
ACAGATCACTAAAGTGGTTCTTAGCAAAGGTTGGAGGTGTCTTGAGTGCA153860.11133605216368295No Hit
GACGGTGGAGTCTGGGGAGGGCGTGTGGGTGGCAGTCAGCGGCGTTTGAG153220.11087293586714872No Hit
TCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAG152420.11029404049648092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)