FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003600016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13819423
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCTC482070.34883511417227764Illumina Single End PCR Primer 1 (96% over 31bp)
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT374460.27096645062532637No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTGCCCCTATGTGTAGATCTC297290.21512475593228456Illumina Single End PCR Primer 1 (96% over 31bp)
TATGTCTGCTGATAATGAAAATGATGACTCTGTCTCAGATGATAACTTTA243810.17642560040314273No Hit
ACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCT235630.17050639523806457No Hit
AGAGGCCCAGAGAGAGAGGTGGTGCTGAGTGTGCAAATCCCCCATCTACT225990.1635307060215177No Hit
ACAGTGTGTACCTTCCAGAACGGTCAACCATGCAGAGTGATGGGAGAGTC220460.15952909177177657No Hit
ACTCCTGTGATGGGCGAGAGGAAGCAGCGATGGGCCGGGCCCCTCCTCCC187600.13575096442159706No Hit
CGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCCAGAGC186710.13510694332172912No Hit
GCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGC182170.13182171209318944No Hit
GGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATA180860.1308737709237209No Hit
CTCTAGTCTAGCCCCCTGCCCCTCAAGCCCACTCTTGCCCCAAGGCCTGG178030.1288259285499836No Hit
CAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTCTTCATTATAGCTATG171200.12388360932290732No Hit
GGGCAGATGGGCAAGACACCTCCAGGTCCTGGCCTGGGCCCTGCTCCTGA168360.12182853075703667No Hit
CAAAGTATTATTACCTGTCCCGAGGCAGCTCCCAGCGAGGGTCTTCGGGA165760.11994712080236636No Hit
CCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAAG163050.11798611273422921No Hit
GAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGA161750.11704540775689404No Hit
AGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGG159150.11516399780222372No Hit
GGCCCACGAAGACTGGTGCCATGATTACCTTCAAGATCTGGACATAAGGC159120.11514228922582367No Hit
CAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGC157990.11432459951475542No Hit
GCAAGGACAGAAGCATCACTTACACTTCAACATCTTCACAGCCACTTTGG155890.11280499916675246No Hit
GACGGTGGAGTCTGGGGAGGGCGTGTGGGTGGCAGTCAGCGGCGTTTGAG152690.11048941768408133No Hit
ACAGATCACTAAAGTGGTTCTTAGCAAAGGTTGGAGGTGTCTTGAGTGCA149810.10840539434967726No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT141540.10242106345539896No Hit
TCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAG139370.10085080976246258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)