FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003600018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11896362
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC193940.16302462887393643Illumina Single End PCR Primer 1 (96% over 31bp)
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT163240.13721842021955954No Hit
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT136140.1144383467819826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)