FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003600019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12051942
Sequences flagged as poor quality0
Sequence length0-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG338070.2805108089634019TruSeq Adapter, Index 6 (97% over 35bp)
CAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATC320500.26593224560821815No Hit
CCAGGTGGTACGTGTGATTGATGGACCCGTATTCATTCTCCACCACACAG312240.2590785783735103No Hit
CAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTA290610.24113126332669044No Hit
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT266590.22120086538750355No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTATG265150.22000603720130746TruSeq Adapter, Index 6 (97% over 35bp)
AAGGCATGGGGTCTGGAGAAAAAACAGAATCATCTCCTGAAGAACAAGAA259810.21557521601083046No Hit
CCACTGGATGTGGGGCTGGGCATCACTGTAAACCTTGCAGACAAACTCTA245650.20382607217990265No Hit
CCACCAGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGC232600.1929979417425009No Hit
AGACAGTGAAACTTGGTACTTCATGGTGAATGTCACAGGTGAGTTGGCCC207680.17232077618694147No Hit
GAGGATACCACATTAGAGCCAGAAGGTAAGTCATTTAATTTCACTTTTCA203460.1688192658079503No Hit
AGAAGCCCCATTTACAATGCAAGGGTAGCTGATTCTAAAACAGGCCTTAA196640.16316042675943845No Hit
ACTCACCATCCTGTGTGCAGGCTCCAAGAAGATTTATGATATTCTTGTGT191220.15866322622528384No Hit
TACAATTAGAGATCTCACTACCTTTTCACTTGGCCAAAAAAATGTAATGG187060.15521150035405085No Hit
AGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAG183990.15266419304042453No Hit
GTTCATAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGA175240.14540395232569156No Hit
TACATTGGTGGTCTTTTTGTAATAGTCTATATTGTTGATATCTCTGGCGA175190.1453624652358931No Hit
TATGTCTGCTGATAATGAAAATGATGACTCTGTCTCAGATGATAACTTTA173730.14415104221377767No Hit
ACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCT171940.1426658043989923No Hit
AGAGGCCCAGAGAGAGAGGTGGTGCTGAGTGTGCAAATCCCCCATCTACT169660.14077399310418187No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT158730.13170491527423547No Hit
CAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTCTTCATTATAGCTATG149010.12363982501741214No Hit
ACAGATCACTAAAGTGGTTCTTAGCAAAGGTTGGAGGTGTCTTGAGTGCA148970.12360663534557335No Hit
ACTCCTGTGATGGGCGAGAGGAAGCAGCGATGGGCCGGGCCCCTCCTCCC148560.12326644120922586No Hit
CAAAGTATTATTACCTGTCCCGAGGCAGCTCCCAGCGAGGGTCTTCGGGA142920.11858669747995801No Hit
CTCTAGTCTAGCCCCCTGCCCCTCAAGCCCACTCTTGCCCCAAGGCCTGG142760.11845393879260288No Hit
CGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCCAGAGC141120.11709316224721295No Hit
GCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGC137960.11447117817194939No Hit
CAAGGAAATCCATTTTTCCCAGGTACATGATGATGAGGGACTGTTGGCAT132560.1099905724737142No Hit
GAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGA131710.10928529194714014No Hit
GGGCAGATGGGCAAGACACCTCCAGGTCCTGGCCTGGGCCCTGCTCCTGA127940.1061571653763352No Hit
CCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAAG125930.10448938436643654No Hit
ACAGTGTGTACCTTCCAGAACGGTCAACCATGCAGAGTGATGGGAGAGTC124270.1031120129851272No Hit
TCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAG123050.10209972799404443No Hit
GGCCCACGAAGACTGGTGCCATGATTACCTTCAAGATCTGGACATAAGGC122620.10174293902177757No Hit
GCAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTACGTGGC122120.10132806812379283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)