FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003600020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12051942
Sequences flagged as poor quality0
Sequence length0-121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATC341710.28353106910073084No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC320120.26561694372574973Illumina Single End PCR Primer 1 (96% over 31bp)
AAGGCATGGGGTCTGGAGAAAAAACAGAATCATCTCCTGAAGAACAAGAA302790.2512375184015987No Hit
CCAGGTGGTACGTGTGATTGATGGACCCGTATTCATTCTCCACCACACAG282780.23463438506424938No Hit
GGACTCTGAAATGACTCCTACAATTGAGGGTTGTGTGAAAGATGTTTCAT250180.20758480251564437No Hit
CAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTA240130.19924589746615112No Hit
CCACCAGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGC240100.199221005212272No Hit
CCACTGGATGTGGGGCTGGGCATCACTGTAAACCTTGCAGACAAACTCTA230540.19128867364280377No Hit
GAGGATACCACATTAGAGCCAGAAGGTAAGTCATTTAATTTCACTTTTCA209270.17364006564253295No Hit
TACAATTAGAGATCTCACTACCTTTTCACTTGGCCAAAAAAATGTAATGG195390.1621232495144766No Hit
AGACAGTGAAACTTGGTACTTCATGGTGAATGTCACAGGTGAGTTGGCCC194650.16150924058545918No Hit
ACTCACCATCCTGTGTGCAGGCTCCAAGAAGATTTATGATATTCTTGTGT189260.15703693230518367No Hit
AGAAGCCCCATTTACAATGCAAGGGTAGCTGATTCTAAAACAGGCCTTAA184530.15311225361024802No Hit
TATGTCTGCTGATAATGAAAATGATGACTCTGTCTCAGATGATAACTTTA180700.14993434253168494No Hit
TACATTGGTGGTCTTTTTGTAATAGTCTATATTGTTGATATCTCTGGCGA178790.14834953570138323No Hit
AGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAG174120.14447464151420578No Hit
GTTCATAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGA169820.140906751791537No Hit
ACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCT168970.14020147126496293No Hit
AGAGGCCCAGAGAGAGAGGTGGTGCTGAGTGTGCAAATCCCCCATCTACT165860.13762097427949785No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT157630.13079219929866903No Hit
ACAGATCACTAAAGTGGTTCTTAGCAAAGGTTGGAGGTGTCTTGAGTGCA143950.11944133152980657No Hit
CGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCCAGAGC143850.11935835735020961No Hit
GAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGA140800.11682764487250269No Hit
ACTCCTGTGATGGGCGAGAGGAAGCAGCGATGGGCCGGGCCCCTCCTCCC139160.11546686832711275No Hit
CTCTAGTCTAGCCCCCTGCCCCTCAAGCCCACTCTTGCCCCAAGGCCTGG138850.11520964837036221No Hit
GCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGC137530.1141143891996825No Hit
CAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTCTTCATTATAGCTATG134320.11145091803462048No Hit
CAAGGAAATCCATTTTTCCCAGGTACATGATGATGAGGGACTGTTGGCAT130840.10856341658464667No Hit
CAAAGTATTATTACCTGTCCCGAGGCAGCTCCCAGCGAGGGTCTTCGGGA130030.1078913257299114No Hit
GGCCCACGAAGACTGGTGCCATGATTACCTTCAAGATCTGGACATAAGGC126720.10514488038525244No Hit
GGGCAGATGGGCAAGACACCTCCAGGTCCTGGCCTGGGCCCTGCTCCTGA125650.10425705666356509No Hit
CCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAAG122480.10162677517034184No Hit
CAGAAGGCTTTGGAGGAGAACAACAACTTCAGCAAGATGGCGGAGGAAAA120610.10007515801187891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)