FastQCFastQC Report
Wed 11 May 2022
EGAF00003600642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32755373
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT16410645.010060486870352Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2949560.9004812737134759No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2113680.645292605887895Illumina Single End PCR Primer 1 (100% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC722840.22067829909920428No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT694950.2121636654847435Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGT532530.16257790744742853Illumina Single End PCR Primer 1 (96% over 31bp)
CGGTGGTCGCCGTATCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT451260.13776671082328995Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTGGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT379990.11600844844599999Illumina Single End PCR Primer 1 (100% over 31bp)
GGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT377210.11515973272537608Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGGGTAGGGAAAGAGT348110.10627569406704664Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)