FastQCFastQC Report
Wed 15 Mar 2023
EGAF00003600654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58821055
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT4596500.7814378711840514TruSeq Adapter, Index 13 (97% over 38bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG1480720.25173298914818854No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC899600.15293843335519908No Hit
CTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATT766930.1303835845854856No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT635100.10797154182290679No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG607670.10330824566135374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)