FastQCFastQC Report
Sun 25 Dec 2022
EGAF00003600659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179923245
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTAT90499735.029907614216273TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATG5490660.30516679487411424TruSeq Adapter, Index 1 (97% over 36bp)
GGCATGGTGATTTCCTTGTATATTGGAATAATTGTGGTAGACATCAATGA4492440.24968647047245063No Hit
GTGATTTCCTTGTATATTGGAATAATTGTGGTAGACATCAATGAGGTTGC4418080.24555359703522464No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG4341220.24128177545930768No Hit
GGTGATTTCCTTGTATATTGGAATAATTGTGGTAGACATCAATGAGGTTG3218470.1788801663731665No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTA2195740.12203759441977605TruSeq Adapter, Index 1 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)