FastQCFastQC Report
Thu 9 Feb 2023
EGAF00003600665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98326796
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAGTAAATCTCGTAT8679780.8827481778212319TruSeq Adapter, Index 6 (97% over 37bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG5953860.6055175437629433No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC2553440.25968912889218926No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT1252340.12736507757254698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)