FastQCFastQC Report
Thu 9 Feb 2023
EGAF00003600670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112611609
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT1667926214.81131665563894TruSeq Adapter, Index 20 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATG7590400.6740335270407157TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTA4986350.4427918261961784TruSeq Adapter, Index 20 (97% over 38bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT1519470.1349301385081888TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATATCGTAT1328510.11797273938249118TruSeq Adapter, Index 20 (97% over 38bp)
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG1181960.10495898340285681No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1168850.10379480502760599No Hit
GTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTT1168480.10376194873478808No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG1153660.10244592100624368No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)