FastQCFastQC Report
Fri 10 Feb 2023
EGAF00003600672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124575020
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATCTCGTAT68258265.4792895076396535TruSeq Adapter, Index 9 (97% over 37bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC6375340.5117671263468391No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT5472000.43925339124970636No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATCTCGTATG5026280.4034741475457921TruSeq Adapter, Index 9 (97% over 36bp)
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC3820710.30669952932778977No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA3792650.30444707133099397No Hit
GCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT2261760.18155806838321198No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT2117000.1699377611980315No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2048350.1644270255786433Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTC1962740.1575548613197092No Hit
GCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGA1862640.1495195425214461No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1766770.1418237781539188No Hit
GTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGG1725220.13848843853286158No Hit
GCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCG1638840.13155446412932545No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAG1443250.11585388467126073No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1325470.10639934073460314No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)