FastQCFastQC Report
Thu 12 May 2022
EGAF00003600681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61105849
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT25810824.2239524402974915Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT4519090.7395511352767556TruSeq Adapter, Index 25 (97% over 38bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG2962460.4848079273065987No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC2600270.42553536896279764No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1233330.20183501582639007Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCA938810.1536366837812858No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA906400.14833277253049867No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGC905650.14821003468915062No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT820590.13428992697573028No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA796140.13028867334778377No Hit
CATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT774440.12673745847144682Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTT691780.11321011185034022TruSeq Adapter, Index 25 (97% over 38bp)
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG682190.11164070398563646No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT651030.10654135580376274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)