FastQCFastQC Report
Wed 11 May 2022
EGAF00003600683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31620085
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT4402201.3922163713348652Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT2674780.8459117045384287TruSeq Adapter, Index 16 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTA775550.24527132042813926TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCGCGTAT510660.16149861709732913TruSeq Adapter, Index 16 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCGCGTA357340.11301044889664275TruSeq Adapter, Index 16 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)