FastQCFastQC Report
Wed 8 Feb 2023
EGAF00003600691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99360842
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT1006421710.12895703923282TruSeq Adapter, Index 15 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATG6543630.6585723176540714TruSeq Adapter, Index 15 (97% over 36bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT3169380.3189767655149299TruSeq Adapter, Index 12 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTA2304170.23189920230345876TruSeq Adapter, Index 15 (97% over 37bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1307110.13155182400728851Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)