FastQCFastQC Report
Thu 23 Feb 2023
EGAF00003600692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99360842
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2463640.24794878449198327Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1367170.13759645877397053Illumina Single End PCR Primer 1 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)