FastQCFastQC Report
Tue 28 Jun 2022
EGAF00003600693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80210290
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT42704795.3241036779694975Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTAT16213982.021433908292814TruSeq Adapter, Index 13 (97% over 38bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG6111350.7619159586631591No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTA3938410.49101056734740645TruSeq Adapter, Index 13 (97% over 38bp)
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC3332740.41550030550943023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCGCGTAT2399590.2991623643300629TruSeq Adapter, Index 13 (97% over 38bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1972040.24585872959691332Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA1760640.21950300890322175No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA1598350.1992699440433391No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCGCGTA1527780.19047182100949892TruSeq Adapter, Index 13 (97% over 38bp)
CCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGC1425820.17776023500226718No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT1311510.16350894629604257No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG1297070.16170867852491247No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGC1154120.14388677562442426No Hit
GGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCA1145400.1427996333138803No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1121060.13976510993788951No Hit
CATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1098570.13696123028603935Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT1037930.12940110302555943No Hit
AATGATACGGCCACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT991910.1236636845472071Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC830110.10349170910615084Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGATACGGCGACCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGT810440.10103940529326101Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)