FastQCFastQC Report
Thu 9 Feb 2023
EGAF00003600695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107193798
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT13127601.2246604043267504TruSeq Adapter, Index 1 (97% over 38bp)
AATGATACGGCGAACTCCAGTCACATCATTCCATCTCGTATGCCGTCTTC2088410.19482563720710783TruSeq Adapter, Index 14 (95% over 22bp)
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG2026760.18907437163482163No Hit
GTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTT1956680.1825366799672496No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1824430.17019921245816852No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT1559020.1454393844688664No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA1556190.14517537665751895No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG1545160.14414639921611883No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG1513920.1412320515035767No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG1457460.1359649557337263No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG1378070.12855874366910666No Hit
CCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGC1361910.12705119376402726No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG1359750.1268496895687939No Hit
GCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGGG1337730.12479546624516466No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA1317340.12289330395775322No Hit
GTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAAT1305620.12179995712065356No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT1305380.12177756776562763No Hit
GTTTGGATGAGAATGGCTGTTACTACGAGGGCTATGTGGCTGATTGAAGA1301550.12142027097500546No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC1293730.1206907511570772No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1285300.11990432506179134No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG1258620.11741537509474195No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG1225890.11436202680308054No Hit
CTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACT1133340.10572813177120566No Hit
GGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGA1126350.1050760418060754No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)