FastQCFastQC Report
Sat 4 Jun 2022
EGAF00003600701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73462605
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT17775032.419602463049057Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10593101.4419717351433425No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6499370.8847181501391083Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2927490.39850070658398246Illumina Single End PCR Primer 1 (100% over 50bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT983400.13386402510501771Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGT788930.10739205341275333Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)