FastQCFastQC Report
Thu 12 May 2022
EGAF00003600704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50066403
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT17968703.5889736276840183Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT5226501.0439136200777195TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA1447830.2891819490207835TruSeq Adapter, Index 1 (97% over 36bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT989850.1977074326669723Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCGCGTAT745560.14891423296377015TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCGCGTA516340.10313103579659996TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)