FastQCFastQC Report
Thu 12 May 2022
EGAF00003600707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003600707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61176907
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT21207623.4666054627443Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4932240.8062257871258514No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2736120.44724719410871816Illumina Single End PCR Primer 1 (100% over 50bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT949190.15515495087059566Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGT800090.13078300934697468Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)