FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003614157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003614157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450595008
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC12387660.2749178259871002Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2418000.080.112189
GAGCGTC2813750.068.873018
ATCGGAA2973650.066.767651
TCGGAAG3092000.063.923752
AGAGCGT3162150.061.4405677
CGGAAGA3276850.060.0416533
AAGAGCG3597050.054.3759466
GAAGAGC6454800.030.8358945
CGCCGTA1741200.022.07007855-59
GGAAGAG9860500.020.5762834
TCGCCGT1868650.020.55626955-59
GCCGTAT1955350.019.74267855-59
GTCGCCG2129400.018.3802155-59
CCGTATC2077700.018.34911755-59
CGTATCA2169550.017.72788460-64
TGGTCGC2248800.017.63285850-54
GTGGTCG2419500.016.53201950-54
GGTCGCC2416750.016.33670250-54
CCGTGTA2522150.015.87581535-39
CGTCGTG2481550.015.83459810-14
Produced by FastQC (version 0.11.5)