FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003614159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003614159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393514223
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGTCTGCGGTGTAGATCTC69049341.7546847347370214Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG9454850.0115.799449
ATCGGAA9894000.0112.2853551
GAGCGTC9773650.0112.006868
AGAGCGT10043500.0109.190047
TCGGAAG10116850.0109.139662
CGGAAGA10351400.0106.568133
AAGAGCG10622450.0103.636466
GAAGAGC13040300.084.645265
GGAAGAG16438200.067.417094
CGCCGTA8434550.025.9541955-59
TCGCCGT8490350.025.69850355-59
GCCGTAT8589350.025.50410555-59
CCGTATC8700050.024.9516755-59
TGGTCGC8866000.024.85557650-54
GTCGCCG8794200.024.66859855-59
CGTATCA8757800.024.59964460-64
GTGGTCG9097400.024.28276850-54
GGTCGCC9045100.024.21960650-54
CGGTGTA9164050.024.21123735-39
AGTGTCG9194800.023.95001825-29
Produced by FastQC (version 0.11.5)