FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003618586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003618586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159009
Sequences flagged as poor quality0
Sequence length71
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGGTTTTGCGCGGTAAC44410.3831721755396205No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGGGCCTAGTCCCCGGATGCTGA38300.33045472468289716No Hit
ATATAGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAATTGAACGTGGGCATT36960.31889312334934417No Hit
ATCCTAAGCTATAGGGAAGTTCCGTTTCAAAGGGGCACTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTA34070.29395802793593495No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGGGCCTAGTCCC30640.26436377974631775No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC25700.2217411599047117No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGG25590.22079207322807676No Hit
CATCAGGATCGGTCGATGATGCGCCGAAGCTCTCACCTACGTTCACTTTCATTACGCGTGCGGGTTTTACA24970.21544267559613425No Hit
GTGTAGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGGTTTTGCGCGGTAACGCAACTGAAC24080.20776370157608784No Hit
ATCCTGATGTATTCGGATGGATTTGAGTAAGAGCGTATAGCCTCGGACCCGAAAGATGGTGAACTATGCCT23630.20388107426258123No Hit
GTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGTACTAACGCCTTTTGTGGTGTC23500.2027594263720126No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTAC23480.20258686515807903No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAGAATTATTCCCTCCC23340.20137893666054363No Hit
GTTATAGCCCGTTGCTTAATACCCTTGTGGGGACCGAGGTTCGCGCTCTGCAAGGACGCTGGCATAATGGT22820.1968923450982693No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTAT21060.18170695827211006No Hit
GCTATAGGGAAGTTCCGTTTCAAAGGGGCACTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTAATATTCC20730.17885969824220518No Hit
AGGCTATATAGCGCCCCCGAGTCCCTAATCATTCGCTTTACCTCATAAAACTGAAGAACAACACTGCTATC20130.17368286182419637No Hit
GCCTATCACCCTGAAAACAGTTTGTCTGGAGATAGGGTTTAATGGCCGGAAGAGCCCAGCACTTCTGCTGG20130.17368286182419637No Hit
CTATATAGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAATTGAACGTGGGCA20100.1734240200032959No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGC19830.17109444361519197No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCTATATTTAGCTTTAGAAGAGATATACCTCCCATTTTGAGCAG19620.16928255086888885No Hit
CTATAGGGAAGTTCCGTTTCAAAGGGGCACTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTAATATTCCG19290.166435290838984No Hit
TTGTTACACAGTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGTACTAACGCCTT18690.16125845442097514No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATG18020.15547765375419864No Hit
TTTCTACCCTGACGGCGGGGTATGGGTGGGACGCTTGAGCGCCATCCATTTTCAGGGCTAGTACATTCGGC17960.15495997011239776No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCTCAAGCGTCCCACCCATACCCC17870.1541834446496964No Hit
GTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCC17860.1540971640427296No Hit
GCTTAATACCCTTGTGGGGACCGAGGTTCGCGCTCTGCAAGGACGCTGGCATAATGGTCATCAGCGACCCG17110.14762611852021856No Hit
GGCTATAACACTCCCGAAGGAGCCACGTTCCCGAAGCTTTTATCCCCCTGCGAGAACCGACGCTGGCCTGA16370.14124135360467435No Hit
GGCCTAGTCCCCGGATGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGCTCCCAGGGGTTCA16290.14055110874893983No Hit
GATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGG16290.14055110874893983No Hit
GGTTAATATTCCGGCACCTGGATGTGGGTTTTGCGCGGTAACGCAACTGAACACGGAGACGACGGCGGGGG16090.13882549660960355No Hit
GAGTACGACCTGGCGTGAGAATTATTCCCTCCCACGGATTTTCAAGGGACGTCGAGAGCGCACCGGACCCA16080.13873921600263672No Hit
GAATTATTCCCTCCCACGGATTTTCAAGGGACGTCGAGAGCGCACCGGACCCAGCAGAAGTGCTGGGCTCT15730.13571939475879824No Hit
CCCTAGGCTTCGTCACTGACCCCCACGGCCGCCTACTCGTCAGGGCATCGTTTCTACCCTGACGGCGGGGT15580.13442518565429604No Hit
GCCATGGGCCTAGTCCCCGGATGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGCTCCCAGG15470.13347609897766108No Hit
ACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAG15040.1297660328780881No Hit
CCCTTATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACC15030.12967975227112127No Hit
GGGCACAAGTGCCCCTTTGAAACGGAACTTCCCTATAGCTTAGGATCGACTAACCCATGTCCAACCGCTGT14940.12890322680841995No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAGAATTATTCCCTCCCACGGATTTTCAAGGG14720.12700505345515006No Hit
GAATAATTCTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAG14670.126573650420316No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA14650.12640108920638235No Hit
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGGGCCT14390.12415779342524519No Hit
GTATTAAGCAACGGGCTATAACACTCCCGAAGGAGCCACGTTCCCGAAGCTTTTATCCCCCTGCGAGAACC14340.12372639039041113No Hit
CTGCTATCCTGAGGGAAACTTCGGCGGTAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCCATGCC14260.12303614553467661No Hit
GTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAGAATTA14220.12269102310680936No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATT13830.11932607943510362No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCGAGGCTATACGCTCTTACTCAAATCCATCCGAATACATCA13420.11578857454946423No Hit
GCTTAGGATCGACTAACCCATGTCCAACCGCTGTTCACATGGAACCTTTCCCCACTTCGGTCCTTCAAGTT13250.1143218042310284No Hit
ATATAGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTA13220.11406296241012795No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAAC13200.11389040119619434No Hit
TCCTTGCAGAGCGCGAACCTCGGTCCCCACAAGGGTATTAAGCAACGGGCTATAACACTCCCGAAGGAGCC13200.11389040119619434No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCTCAAGCGTCCCA13190.11380412058922752No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG13050.11259619209169212No Hit
CTTCTGCTGGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGTGGGAGGGAATAATTCTCACGCCAGGT13050.11259619209169212No Hit
TCGCTACACGGGGCACAAGTGCCCCTTTGAAACGGAACTTCCCTATAGCTTAGGATCGACTAACCCATGTC12820.11061173813145542No Hit
CCTTAACCCCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGTGTTGGTA12710.10966265145482045No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACA12700.10957637084785365No Hit
GCACTAGGGGCCGTTCGACCCGGGCTCACGCCCTAGGCTTCGTCACTGACCCCCACGGCCGCCTACTCGTC12640.10905868720605275No Hit
GAGTAAGAGCGTATAGCCTCGGACCCGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAAC12530.1081096005294178No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGA12440.1073330750667165No Hit
GGGTATGGGTGGGACGCTTGAGCGCCATCCATTTTCAGGGCTAGTACATTCGGCAGGTGAGTTGTTACACA12210.10534862110647976No Hit
CCCTTAGGGGTATCGCATACTAAATTCATAGGTATGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAG12000.10353672836017667No Hit
GCTATATAGCGCCCCCGAGTCCCTAATCATTCGCTTTACCTCATAAAACTGAAGAACAACACTGCTATCCT11940.10301904471837578No Hit
GATCTGCACTAGGGGCCGTTCGACCCGGGCTCACGCCCTAGGCTTCGTCACTGACCCCCACGGCCGCCTAC11850.10224251925567446No Hit
GGGTTAAGGTGCCAGAGTGGACGCTCATCAGACACCACAAAAGGCGTTAGTACATCTTGACAGCAGGACGG11830.10206995804174082No Hit
GCCCAGCACTTCTGCTGGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGTGGGAGGGAATAATTCTCA11710.10103459075813906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)