FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003618587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003618587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159009
Sequences flagged as poor quality0
Sequence length71
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGGTTTTGCGCGGTAAC42670.36815934992739485No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGGGCCTAGTCCCCGGATGCTGA37450.323120873090718No Hit
ATATAGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAATTGAACGTGGGCATT35720.3081943280854592No Hit
ATCCTAAGCTATAGGGAAGTTCCGTTTCAAAGGGGCACTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTA33280.28714185998555664No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGGGCCTAGTCCC29140.25142168870129566No Hit
ATCCTGATGTATTCGGATGGATTTGAGTAAGAGCGTATAGCCTCGGACCCGAAAGATGGTGAACTATGCCT25400.2191527416957073No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC24780.2138033440637648No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTAC23390.2018103396953777No Hit
GTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGTACTAACGCCTTTTGTGGTGTC23360.20155149787447726No Hit
GTGTAGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGGTTTTGCGCGGTAACGCAACTGAAC23160.19982588573514096No Hit
GTTATAGCCCGTTGCTTAATACCCTTGTGGGGACCGAGGTTCGCGCTCTGCAAGGACGCTGGCATAATGGT23080.19913564087940647No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGG22330.1926645953568954No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCTCAAGCGTCCCACCCATACCCC21670.1869700752970857No Hit
CATCAGGATCGGTCGATGATGCGCCGAAGCTCTCACCTACGTTCACTTTCATTACGCGTGCGGGTTTTACA21210.18300116737661226No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTAT21090.1819658000930105No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAGAATTATTCCCTCCC20710.17868713702827158No Hit
GGGCACAAGTGCCCCTTTGAAACGGAACTTCCCTATAGCTTAGGATCGACTAACCCATGTCCAACCGCTGT20420.17618499942623395No Hit
CTATATAGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAATTGAACGTGGGCA20370.1757535963913999No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGC19680.16980023451068973No Hit
GCTATAGGGAAGTTCCGTTTCAAAGGGGCACTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTAATATTCC19110.16488223991358134No Hit
GCCTATCACCCTGAAAACAGTTTGTCTGGAGATAGGGTTTAATGGCCGGAAGAGCCCAGCACTTCTGCTGG19100.16479595930661453No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCTATATTTAGCTTTAGAAGAGATATACCTCCCATTTTGAGCAG18930.1633291889881787No Hit
AGGCTATATAGCGCCCCCGAGTCCCTAATCATTCGCTTTACCTCATAAAACTGAAGAACAACACTGCTATC18130.15642674043083357No Hit
CTATAGGGAAGTTCCGTTTCAAAGGGGCACTTGTGCCCCGTGTAGCGAAAGGGAAGCCGGTTAATATTCCG17900.15444228647059688No Hit
GGCTATAACACTCCCGAAGGAGCCACGTTCCCGAAGCTTTTATCCCCCTGCGAGAACCGACGCTGGCCTGA17820.15375204161486236No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATG17670.15245783251036013No Hit
TTGTTACACAGTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGTACTAACGCCTT17170.14814380216201944No Hit
GTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCC16540.14270812392311016No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCTCAAGCGTCCCA16520.14253556270917656No Hit
GCTTAATACCCTTGTGGGGACCGAGGTTCGCGCTCTGCAAGGACGCTGGCATAATGGTCATCAGCGACCCG16430.14175903724647523No Hit
TTTCTACCCTGACGGCGGGGTATGGGTGGGACGCTTGAGCGCCATCCATTTTCAGGGCTAGTACATTCGGC16000.13804897114690223No Hit
GGCCTAGTCCCCGGATGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGCTCCCAGGGGTTCA15710.1355468335448646No Hit
GAGTACGACCTGGCGTGAGAATTATTCCCTCCCACGGATTTTCAAGGGACGTCGAGAGCGCACCGGACCCA15660.13511543051003055No Hit
CCCTTATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACC15430.13313097654979383No Hit
GATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGG15250.13157792562439116No Hit
GAATAATTCTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAG15020.12959347166415447No Hit
GTATTAAGCAACGGGCTATAACACTCCCGAAGGAGCCACGTTCCCGAAGCTTTTATCCCCCTGCGAGAACC14870.12829926255965224No Hit
GGTTAATATTCCGGCACCTGGATGTGGGTTTTGCGCGGTAACGCAACTGAACACGGAGACGACGGCGGGGG14860.12821298195268546No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA14780.12752273709695094No Hit
GTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAGAATTA14740.12717761466908367No Hit
GAATTATTCCCTCCCACGGATTTTCAAGGGACGTCGAGAGCGCACCGGACCCAGCAGAAGTGCTGGGCTCT14440.12458919646007925No Hit
CTGCTATCCTGAGGGAAACTTCGGCGGTAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCCATGCC14330.12364010978344431No Hit
GGGTTAAGGTGCCAGAGTGGACGCTCATCAGACACCACAAAAGGCGTTAGTACATCTTGACAGCAGGACGG14240.12286358432074297No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAGAATTATTCCCTCCCACGGATTTTCAAGGG14220.12269102310680936No Hit
ACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAG14110.12174193643017439No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATT14010.12087913036050627No Hit
GCTTAGGATCGACTAACCCATGTCCAACCGCTGTTCACATGGAACCTTTCCCCACTTCGGTCCTTCAAGTT13950.12036144671870538No Hit
GCCATGGGCCTAGTCCCCGGATGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGCTCCCAGG13950.12036144671870538No Hit
CCCTAGGCTTCGTCACTGACCCCCACGGCCGCCTACTCGTCAGGGCATCGTTTCTACCCTGACGGCGGGGT13770.11880839579330273No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGA13640.11768674790273415No Hit
TCCTTGCAGAGCGCGAACCTCGGTCCCCACAAGGGTATTAAGCAACGGGCTATAACACTCCCGAAGGAGCC13620.11751418668880051No Hit
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGAGCGGCCGAAGCCTGCCATGGGCCT13510.11656510001216557No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG12830.11069801873842224No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCGAGGCTATACGCTCTTACTCAAATCCATCCGAATACATCA12730.10983521266875408No Hit
TCGCTACACGGGGCACAAGTGCCCCTTTGAAACGGAACTTCCCTATAGCTTAGGATCGACTAACCCATGTC12580.10854100356425186No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACA12540.10819588113638462No Hit
CTTCTGCTGGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGTGGGAGGGAATAATTCTCACGCCAGGT12540.10819588113638462No Hit
ATATAGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTA12120.10457209564377844No Hit
GGATAGCAGTGTTGTTCTTCAGTTTTATGAGGTAAAGCGAATGATTAGGGACTCGGGGGCGCTATATAGCC11980.10336416714624304No Hit
GAGTAAGAGCGTATAGCCTCGGACCCGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAAC11860.10232879986264128No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAAAC11690.10086202954420542No Hit
TCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAAC11620.10025806529543775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)