FastQCFastQC Report
Tue 28 Jun 2022
EGAF00003619143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133464597
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4246660.31818625279331564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGTGTGTGTGTGTGTGTG2421220.18141290307870933Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG1805990.13531603440873538No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC1683760.12615780048397404No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC1516640.1136361277890046No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC1378340.10327382923877558No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG1366270.10236946956053072No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1342630.10059821332244386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)