FastQCFastQC Report
Sat 2 Jul 2022
EGAF00003619147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132338748
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4427300.3345429866088804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGTGTGTGTGTGTGTGTG2270430.1715619978511509Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG1827810.13811601119273095No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC1695650.12812951804561426No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC1500690.11339762712580598No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC1394220.10535236437328242No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG1372250.10369223078942834No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1333290.10074827064254831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)