FastQCFastQC Report
Thu 16 Jun 2022
EGAF00003619285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138168407
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9055870.6554226249420391No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGTGTGTGTGTGTGTGTG6247670.4521778991054011Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTGTGTGTGTGTGTGTGT3005340.2175128211473119Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1586320.11481061658328304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)