FastQCFastQC Report
Wed 11 May 2022
EGAF00003619304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143917954
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA4258273929.588204818420362No Hit
GCTGAATT3780129026.265861172540017No Hit
ATGCTCCG3368617223.406511184837996No Hit
TGAAGTAC2839537519.730252001775956No Hit
CACTCGAA1637690.11379330753965554RNA PCR Primer, Index 47 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)