FastQCFastQC Report
Wed 11 May 2022
EGAF00003619309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144687719
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA4283366029.604212642263022No Hit
GCTGAATT3814482726.363555430713507No Hit
ATGCTCCG3380770823.365983121207407No Hit
TGAAGTAC2863740819.792563043999607No Hit
CACTCGAA1660840.11478790401001483RNA PCR Primer, Index 47 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)