FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761
Sequences flagged as poor quality0
Sequence length140
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT511.0712035286704475No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC330.6931316950220542No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC310.6511237135055661No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG210.4410838059231254No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA200.42007981516488135No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC200.42007981516488135No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG190.39907582440663725No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT180.3780718336483932No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA180.3780718336483932No Hit
TGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGC180.3780718336483932No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG180.3780718336483932No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG180.3780718336483932No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA180.3780718336483932No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT170.35706784289014915No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC160.33606385213190504No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG160.33606385213190504No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC160.33606385213190504No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA150.315059861373661No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG150.315059861373661No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC150.315059861373661No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT150.315059861373661No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT150.315059861373661No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG140.29405587061541694No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC140.29405587061541694No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC140.29405587061541694No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC140.29405587061541694No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG140.29405587061541694No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA140.29405587061541694No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG130.2730518798571729No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA130.2730518798571729No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT130.2730518798571729No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG130.2730518798571729No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC120.2520478890989288No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT120.2520478890989288No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG120.2520478890989288No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT120.2520478890989288No Hit
GGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTC110.23104389834068476No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA110.23104389834068476No Hit
GGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAA110.23104389834068476No Hit
TCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAG110.23104389834068476No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT100.21003990758244068No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA100.21003990758244068No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT100.21003990758244068No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC90.1890359168241966No Hit
GGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACA90.1890359168241966No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG90.1890359168241966No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA90.1890359168241966No Hit
GCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCT90.1890359168241966No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT90.1890359168241966No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT80.16803192606595252No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG80.16803192606595252No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC80.16803192606595252No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC80.16803192606595252No Hit
TGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTC80.16803192606595252No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA80.16803192606595252No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC80.16803192606595252No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG80.16803192606595252No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA80.16803192606595252No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC80.16803192606595252No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA80.16803192606595252No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT80.16803192606595252No Hit
CACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAG80.16803192606595252No Hit
TCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC80.16803192606595252No Hit
CCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACT70.14702793530770847No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT70.14702793530770847No Hit
GGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCT70.14702793530770847No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA70.14702793530770847No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT70.14702793530770847No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG70.14702793530770847No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG70.14702793530770847No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG70.14702793530770847No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA70.14702793530770847No Hit
GACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCA70.14702793530770847No Hit
CCCTAGAGGCCTAGGCTGGCTGTCTTCAGGTCAGGGGGCTGAGTTGCCAT70.14702793530770847No Hit
GTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGA70.14702793530770847No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT70.14702793530770847No Hit
TGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATG60.1260239445494644No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC60.1260239445494644No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC60.1260239445494644No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC60.1260239445494644No Hit
CAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGG60.1260239445494644No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA60.1260239445494644No Hit
GGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGC60.1260239445494644No Hit
CTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACC60.1260239445494644No Hit
GTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGC60.1260239445494644No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC60.1260239445494644No Hit
CACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGT60.1260239445494644No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC60.1260239445494644No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC60.1260239445494644No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT60.1260239445494644No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA60.1260239445494644No Hit
CTCATGTACTGCTGGGGCACAGTGAGCAGAGAGGTGCCTGTGTCCACGAT60.1260239445494644No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA60.1260239445494644No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT60.1260239445494644No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT60.1260239445494644No Hit
GTATCAACGCAGAGTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAG50.10501995379122034No Hit
CCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAA50.10501995379122034No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA50.10501995379122034No Hit
TAGTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGT50.10501995379122034No Hit
CTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCC50.10501995379122034No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG50.10501995379122034No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT50.10501995379122034No Hit
CACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTG50.10501995379122034No Hit
GCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTG50.10501995379122034No Hit
AGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTG50.10501995379122034No Hit
ACGCAGAGTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATG50.10501995379122034No Hit
GCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATC50.10501995379122034No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT50.10501995379122034No Hit
GGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC50.10501995379122034No Hit
CCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTG50.10501995379122034No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG50.10501995379122034No Hit
AAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGC50.10501995379122034No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG50.10501995379122034No Hit
GGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA50.10501995379122034No Hit
GGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGG50.10501995379122034No Hit
ACGCAGAGTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGG50.10501995379122034No Hit
CTTCCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGG50.10501995379122034No Hit
TATCAACGCAGAGTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAA50.10501995379122034No Hit
CCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGGA50.10501995379122034No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG50.10501995379122034No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA50.10501995379122034No Hit
TCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGC50.10501995379122034No Hit
TTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAG50.10501995379122034No Hit
CCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTG50.10501995379122034No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG50.10501995379122034No Hit
GCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGG50.10501995379122034No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT50.10501995379122034No Hit
ACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGG50.10501995379122034No Hit
GGCTGCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACC50.10501995379122034No Hit
GTACATGGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGC50.10501995379122034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)