FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761
Sequences flagged as poor quality0
Sequence length140
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC420.8821676118462508No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT320.6721277042638101No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT270.5671077504725899No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC210.4410838059231254No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG210.4410838059231254No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA200.42007981516488135No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT200.42007981516488135No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC190.39907582440663725No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA180.3780718336483932No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT180.3780718336483932No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG170.35706784289014915No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG160.33606385213190504No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG160.33606385213190504No Hit
TGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGC150.315059861373661No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT150.315059861373661No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT150.315059861373661No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG140.29405587061541694No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC140.29405587061541694No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA140.29405587061541694No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA140.29405587061541694No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA130.2730518798571729No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC130.2730518798571729No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG130.2730518798571729No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC130.2730518798571729No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT130.2730518798571729No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT130.2730518798571729No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG120.2520478890989288No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA120.2520478890989288No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC110.23104389834068476No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC110.23104389834068476No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT110.23104389834068476No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC110.23104389834068476No Hit
TTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAG110.23104389834068476No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG110.23104389834068476No Hit
CCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTG110.23104389834068476No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG110.23104389834068476No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT100.21003990758244068No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC100.21003990758244068No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG100.21003990758244068No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG100.21003990758244068No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC100.21003990758244068No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA100.21003990758244068No Hit
CATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAG100.21003990758244068No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG90.1890359168241966No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT90.1890359168241966No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG90.1890359168241966No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT90.1890359168241966No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG90.1890359168241966No Hit
GAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGC90.1890359168241966No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA90.1890359168241966No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG90.1890359168241966No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT80.16803192606595252No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC80.16803192606595252No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA80.16803192606595252No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC80.16803192606595252No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA80.16803192606595252No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC80.16803192606595252No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG80.16803192606595252No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA80.16803192606595252No Hit
CGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCG80.16803192606595252No Hit
GGAATGCTCTCTGCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCC80.16803192606595252No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA80.16803192606595252No Hit
TCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACC80.16803192606595252No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT70.14702793530770847No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT70.14702793530770847No Hit
CACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTG70.14702793530770847No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG70.14702793530770847No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA70.14702793530770847No Hit
GCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACG70.14702793530770847No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC70.14702793530770847No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG70.14702793530770847No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG70.14702793530770847No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT70.14702793530770847No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC70.14702793530770847No Hit
AGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCA70.14702793530770847No Hit
CCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGC70.14702793530770847No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT70.14702793530770847No Hit
TGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATG70.14702793530770847No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT70.14702793530770847No Hit
CACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTC70.14702793530770847No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT70.14702793530770847No Hit
TGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATG60.1260239445494644No Hit
CTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGG60.1260239445494644No Hit
CTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCC60.1260239445494644No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA60.1260239445494644No Hit
GCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCA60.1260239445494644No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT60.1260239445494644No Hit
CCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGA60.1260239445494644No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC60.1260239445494644No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA60.1260239445494644No Hit
GACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCA60.1260239445494644No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA60.1260239445494644No Hit
GCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGC60.1260239445494644No Hit
GTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCA60.1260239445494644No Hit
GCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTT60.1260239445494644No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC60.1260239445494644No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT60.1260239445494644No Hit
CAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCT60.1260239445494644No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT60.1260239445494644No Hit
GCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGG60.1260239445494644No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT60.1260239445494644No Hit
TGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTC60.1260239445494644No Hit
CTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAAC50.10501995379122034No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG50.10501995379122034No Hit
GCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCAC50.10501995379122034No Hit
GGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTC50.10501995379122034No Hit
GGGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG50.10501995379122034No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC50.10501995379122034No Hit
GCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGAC50.10501995379122034No Hit
TGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCACTCCCATCCT50.10501995379122034No Hit
CTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGC50.10501995379122034No Hit
TCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTG50.10501995379122034No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT50.10501995379122034No Hit
TGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCATCCT50.10501995379122034No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC50.10501995379122034No Hit
CTGCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTG50.10501995379122034No Hit
TCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTG50.10501995379122034No Hit
TGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACAC50.10501995379122034No Hit
GGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA50.10501995379122034No Hit
GATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGC50.10501995379122034No Hit
CCCCTGGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATT50.10501995379122034No Hit
ACGCAGAGTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGG50.10501995379122034No Hit
GTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTT50.10501995379122034No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT50.10501995379122034No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA50.10501995379122034No Hit
GGCTTCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGG50.10501995379122034No Hit
GAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCC50.10501995379122034No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT50.10501995379122034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)