FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003619337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003619337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.25220680958385877No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA50.2101723413198823No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC50.2101723413198823No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40.16813787305590583Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAAGGGGATATCCACAGAGTACCTTGTGGGCATTAGGTGATTGTAGT40.16813787305590583No Hit
GAGTAGTAGCGGGCCTGCAGGCGCGGCAGCAGCTCACACAGGTGGTCGAT40.16813787305590583No Hit
ATAGGGTTCATGTTATAGGATTCAATTGTAACATTAGTTGGTGTAGGCAC40.16813787305590583No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA40.16813787305590583No Hit
GCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCG40.16813787305590583No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA40.16813787305590583No Hit
CAGGAGAATCGCTTGAACCCGGTACACGGAGGTTGTGGTGAACCCAGATC40.16813787305590583No Hit
AGGTCATTCCCTGAGTCAGACATGCCTCTTTGACTACCGTGTGCCAGTGC40.16813787305590583No Hit
TTTTGAGTCTTTTCCATTCTGATTTGATTTTTGTGCATTTTTGGCTGGAG30.12610340479192939No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG30.12610340479192939No Hit
CCCTCCTGCATCTGCCACCCGGGTTACCATGGAGAGAGGTGTCATGGGCT30.12610340479192939No Hit
ACCCTATGTATTCAGATAACGATACCTTTCGGTGGGTTTCCCCATTCGGA30.12610340479192939No Hit
TGGGGGCACAGCTAAACCATATCACACTTTTAATGTCACTGTATTCCTGG30.12610340479192939No Hit
CACTGAGAACAACGTCAGCTCTCCTGGTTTTCCTGTCACTGGTAGACCCT30.12610340479192939No Hit
AGTTAAGGTTGATACCATATGAGAAATATTTTTGCTACAGTATCAAATTA30.12610340479192939No Hit
ATCCAGAGCGGCCTGGGCGCTCTGAGTCGCAGCCACGACACCACCAGCAA30.12610340479192939No Hit
CACCTGGTGCTCCGTCTCAGAGGTGGGATGCAGATCTTCGTGAAGACCCT30.12610340479192939No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG30.12610340479192939No Hit
CTTCTGGAGTCACATGATTGGTAGCTGCTTTAATGTTCTTCAATCTCTCT30.12610340479192939No Hit
AAGCAAGACTCTGTCTTTAAAAAAAAAAAAAAAGAACAGGCCAGGTGCGG30.12610340479192939No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGG30.12610340479192939No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC30.12610340479192939No Hit
GATGGGGATGGTGGGCTGGGAAGATGGCTCCCATCTCAGGGTGAGGGGCT30.12610340479192939No Hit
GTCCTCACTGACAGCTGCCAGAAGCAGATCTTTGGTCTCATTTGAAATAG30.12610340479192939No Hit
GCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGG30.12610340479192939No Hit
CCCCAGAACCATGCGACACCCACGATAGCCACTGGCCAGGTGCCCGCCGT30.12610340479192939No Hit
GGCTCGTTGATGATCCGCAGCACGTTGAGCCCCGCGATCACACCCGCATC30.12610340479192939No Hit
ATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAA30.12610340479192939No Hit
ACCTCAGATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGG30.12610340479192939No Hit
GCCCAGAATGACAAGCATACTGGCCCAGTGAGAGCCTTGGATGTGAACAT30.12610340479192939No Hit
CCACCATTCTCTGCTGCATCGCCTTCTTCATCTTCGTGCTCCAGCTCTTC30.12610340479192939No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30.12610340479192939No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG30.12610340479192939No Hit
GGCACCATCTTGGCTCACCGCAACCTCTGCCTCCTGGGTTCAAGTGATTC30.12610340479192939No Hit
GAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC30.12610340479192939No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG30.12610340479192939No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGGTGGTGCATGGC30.12610340479192939No Hit
TTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATC30.12610340479192939No Hit
GACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG30.12610340479192939No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA30.12610340479192939No Hit
CCATTTATACAGACAGGCGTGAAGACATTCACGACAAAAACGCGAAATTC30.12610340479192939No Hit
GTATAAAGAGAACTAAATCTATACACAGCTGTGCCATAAGAGCATTAGGT30.12610340479192939No Hit
GTTTATAGGAGTTCTTATTTTAGTATTTTTGCCATTTTCCTGTAAGTGTG30.12610340479192939No Hit
GGTTTAGGATATGCTGAGTAGATTCTTGCTTGATTCCTCTATGAGGTGAA30.12610340479192939No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG30.12610340479192939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA30.12610340479192939No Hit
CTGTAGTAATCCAGGCAAGAGGTGGCAAGGAAGGGACATGTTGGAGACGC30.12610340479192939No Hit
GTGCACTCACTGGGGACGCTGTCCTGCATCTGACGCCCAAGGTGCTGGGG30.12610340479192939No Hit
TACAGTGGGAGGGTCCGGGTTGCTGGTTCCAGCAGCTAGCCCTCTCCGAA30.12610340479192939No Hit
TTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTAC30.12610340479192939No Hit
GTGCAATGGCACAATCTCGGCTCACTGCAAGCTCTGGGTTCACACCATTC30.12610340479192939No Hit
GACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAA30.12610340479192939No Hit
GTCGCGGCCGCCTTCTCATCGCTCCTGGAAGGTCCCGAGCGCGACACCAT30.12610340479192939No Hit
CTACACTACTCCTGAGGATGCTACTCCCGAGCCCGGAGAGGACCCACGCG30.12610340479192939No Hit
GTTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCA30.12610340479192939No Hit
ATATGTTTCTTGGGTATCACCAGAAAATGTGTTGGTGCTTGAGGGGAAAT30.12610340479192939No Hit
ATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCC30.12610340479192939No Hit
CTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACT30.12610340479192939No Hit
TCTTCGTGCTGGGCGCTGAGGATGGCTGCATCAGCACCAAGGAGCTGGGC30.12610340479192939No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT30.12610340479192939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)